SIMULTANEOUS IDENTIFICATION AND QUANTITATION OF CODEINE, MORPHINE, HYDROCODONE, AND HYDROMORPHONE IN URINE AS TRIMETHYLSILYL AND OXIME DERIVATIVES BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

Following enzymatic hydrolysis of urine, a gas chromatography-mass spe ctrometry method for the simultaneous determination of codeine, morphi ne, hydrocodone, and hydromorphone uses hydroxylamine to form oxime de rivatives of the keto-opiates (i.e., hydrocodone, hydromorphone, oxyco done, and oxymorphone). These trimethylsilyl-derivatized forms no long er interfere with the detection and quantitation of codeine and morphi ne. Samples are extracted on solid-phase columns and quantitated by de uterated internal calibrations of each analyte with selected ion monit oring. Codeine, morphine, hydrocodone, and hydromorphone are completel y separated, allowing simultaneous quantitation without interference a nd a chromatographic analysis time <9 min.