Molecular characterisation of In51, a class 1 integron containing a novel aminoglycoside adenylyltransferase gene cassette, aadA6, in Pseudomonas aeruginosa

Authors
Naas, T Poirel, L Nordmann, P
Citation
T. Naas et al., Molecular characterisation of In51, a class 1 integron containing a novel aminoglycoside adenylyltransferase gene cassette, aadA6, in Pseudomonas aeruginosa, BBA-GENE ST, 1489(2-3), 1999, pp. 445-451
Citations number
24
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1489
Issue
2-3
Year of publication
1999
Pages
445 - 451
Database
ISI
SICI code
0167-4781(199912)1489:2-3<445:MCOIAC>2.0.ZU;2-8
Abstract
Polymerase chain reaction-amplification and subsequent sequencing of the va riable region of a novel integron, In51, from Pseudomonas aeruginosa reveal ed the presence of a novel aminoglycoside adenylyltransferase gene, aadA6, together with an open reading frame of unknown function, orfD. AADA6 enzyme has only 75% amino acid identity with AADA1 and is able to confer high lev el resistance to streptomycin and spectinomycin in Escherichia coli. (C) 19 99 Elsevier Science B.V. All rights reserved.