Sr. Sturzenbaum et al., Characterisation and quantification of earthworm cyclophilins: identification of invariant and heavy metal responsive isoforms, BBA-GENE ST, 1489(2-3), 1999, pp. 467-473
Citations number
25
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
We report the identification of two earthworm cyclophilin genes, which rese
mble the cytosolic cyclophilin-A and the signal sequence containing cycloph
ilin-B. Using fully quantitative PCR we were able to assess the transcript
regulation of both cyclophilin isoforms, as well as a further independent c
ontrol gene (actin), during exposure to heavy metals. Whilst the expression
of cyclophilin-B and actin remained exceptionally constant, cyclophilin-A
was up-regulated 38-fold. This intriguing observation has profound implicat
ions regarding cyclophilin's use as an invariant control and highlights the
fact that it is essential to treat cyclophilin isoforms as separate entiti
es, rather than one functional unit. (C) 1999 Elsevier Science B.V. All rig
hts reserved.