Nuclear microscopy in the life sciences at the National University of Singapore - A review

The nuclear microscope is now gaining popularity in the field of life scien ces. in particular, the combination of proton-induced X-ray emission to mea sure the elemental concentrations of inorganic elements, Rutherford backsca ttering spectrometry to characterize the organic matrix, and scanning trans mission ion microscopy to provide information on the density and structure of the sample represents a powerful set of techniques that can be applied s imultaneously to the specimen under investigation. These techniques are ext remely useful for measuring any imbalances in trace elements in localized r egions of biological tissue and, as such, can provide unique information on many diseases. Zn this article, we describe the nuclear microscope and its related ion-beam techniques, and we review the biomedical work carried out using the nuclear microscope in the National University of Singapore.