1,1-dimethylallylcoumarins potently supress both lipopolysaccharide- and interferon-gamma-induced nitric oxide generation in mouse macrophage RAW 264.7 cells

Authors
Murakami, A Gao, GX Omura, M Yano, M Ito, C Furukawa, H Takahashi, D Koshimizu, K Ohigashi, H
Citation
A. Murakami et al., 1,1-dimethylallylcoumarins potently supress both lipopolysaccharide- and interferon-gamma-induced nitric oxide generation in mouse macrophage RAW 264.7 cells, BIOORG MED, 10(1), 2000, pp. 59-62
Citations number
20
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
10
Issue
1
Year of publication
2000
Pages
59 - 62
Database
ISI
SICI code
0960-894X(20000103)10:1<59:1PSBLA>2.0.ZU;2-5
Abstract
We investigated the suppressive effects of 16 coumarin-related compounds on both lipopolysaccharide (LPS)- and interferon (IFN)-gamma-induced nitric o xide (NO) generation in a mouse macrophage cell line, RAW 264.7. Notably, c oumarins possessing prenyl unit(s) were found to be highly active, a tenden cy consistent with our previous study. Among the coumarins tested, 1,1-dime thylallylcoumarins showed the highest inhibitory activity. Western blotting analysis revealed that they inhibited NO generation by suppressing inducib le NO synthase (iNOS) protein expression. Our ongoing studies suggest that coumarins are prominent natural compounds that attenuate excessive and prol onged NO generation at inflammatory sites. (C) 1999 Elsevier Science Ltd. A ll rights reserved.