The use of the replicating pDblet plasmid as a cloning vector with enhanced stability in Kluyveromyces marxianus

Authors
de Souza, CG de Morais, MA
Citation
Cg. De Souza et Ma. De Morais, The use of the replicating pDblet plasmid as a cloning vector with enhanced stability in Kluyveromyces marxianus, BIOTECH LET, 22(1), 2000, pp. 43-45
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
1
Year of publication
2000
Pages
43 - 45
Database
ISI
SICI code
0141-5492(200001)22:1<43:TUOTRP>2.0.ZU;2-M
Abstract
The replicating plasmid, pDblet, transformed the budding yeast Kluyveromyce s marxianus to an efficiency of 10(4) transformant mu g(-1) DNA. Transforme d cells showed 1% of segregation rate without affecting their growth rate o f 0.69 h(-1) and glucose consumption. These results were similar or better than the commonly used pE1 plasmid and suggests that pDblet can be used for cloning genes in K. marxianus.