Cg. De Souza et Ma. De Morais, The use of the replicating pDblet plasmid as a cloning vector with enhanced stability in Kluyveromyces marxianus, BIOTECH LET, 22(1), 2000, pp. 43-45
The replicating plasmid, pDblet, transformed the budding yeast Kluyveromyce
s marxianus to an efficiency of 10(4) transformant mu g(-1) DNA. Transforme
d cells showed 1% of segregation rate without affecting their growth rate o
f 0.69 h(-1) and glucose consumption. These results were similar or better
than the commonly used pE1 plasmid and suggests that pDblet can be used for
cloning genes in K. marxianus.