Optimum ratio of D-carbamoylase to D-hydantoinase for maximizing D-p-hydroxyphenylglycine productivity

By use of PCR, the genes encoding D-carbamoylase and D-hydantoinase, respon sible for the synthesis of D-p-hydroxyphenylglycine (d-HPG), were cloned se parately from Agrobacterium radiobacter NRRL B11291 into Escherichia coli. D-HPG production was carried out with a mixed population consisting of vari ous proportions of two recombinants producing individual enzymes. As a resu lt, with a total amount of 192 mg dry cell, the optimal productivity of d-H PG (ca. 4.5 g h(-1) l(-1)) was obtained when the ratio of D-carbamoylase to D-hydantoinase ranged between 1 and 2.