Immobilization of native and dextran-free dextransucrases from Leuconostocmesenteroides NRRL B-512F for the synthesis of glucooligosaccharides

Dextransucrase from Leuconostoc mesenteroides NRRL B-512F was immobilized u sing two different methods: covalent attachment to activated silica and ent rapment in calcium alginate. For immobilization on silica, native enzyme an d dextran-free enzyme were compared. However, the entrapment in calcium alg inate beads gave the best results in terms of immobilization yield and stab ility. This biocatalyst was employed in the acceptor reaction with maltose showing similar glucooligosaccharide production than the native enzyme but increased operational stability.