Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells

Authors
Chang, KH Park, CH Park, JH Chung, IS
Citation
Kh. Chang et al., Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells, BIOTECH TEC, 13(11), 1999, pp. 757-759
Citations number
9
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
11
Year of publication
1999
Pages
757 - 759
Database
ISI
SICI code
0951-208X(199911)13:11<757:OCDAMO>2.0.ZU;2-9
Abstract
The human rotavirus titer was optimal at an infection cell density of about 4-8 x 10(4) cells cm(-2) in monkey kidney cell cultures. The highest viral titers (3.8 x 10(7) TCID50 ml(-1) and 3.7 x 10(7) TCID50 ml(-1)) were obta ined at an multiplicity of infection of 0.05 in well plate and T-flask, res pectively.