The synthesis and evaluation of novel sialic acid analogues bound to matrices for the purification of sialic acid-recognising proteins

Citation
S. Abo et al., The synthesis and evaluation of novel sialic acid analogues bound to matrices for the purification of sialic acid-recognising proteins, CARBOHY RES, 322(3-4), 1999, pp. 201-208
Citations number
18
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
322
Issue
3-4
Year of publication
1999
Pages
201 - 208
Database
ISI
SICI code
0008-6215(199912)322:3-4<201:TSAEON>2.0.ZU;2-Z
Abstract
A novel N-acetylneuraminic acid analogue, 2-S-(5'-aminopentyl) 5-acetamido- 3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-2-nonulopyranosidonic acid, as well as the thiosialoside 2-S-(2'-aminoethyl) 5-acetamido-3,5-dideoxy-2-th io-D-glycero-alpha-D-galacto-2-nonulopyranosidonic acid, have been synthesi sed and successfully coupled to CNBr-activated Sepharose 4B through the ter minal amino group. The resultant affinity resins have proved efficient in p urifying a number of sialic acid-recognising proteins such as Vibrio choler ae sialidase, sialidase-l from leech, trans-sialidase from Trypanosoma cruz i, and sialyltransferases from rat liver, all in high yield. (C) 1999 Elsev ier Science Ltd. All rights reserved.