Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice

Authors
Wang, XY Xing, H He, QH Xu, JL Wu, BJ
Citation
Xy. Wang et al., Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice, CHIN SCI B, 45(1), 2000, pp. 49-51
Citations number
6
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
10016538 → ACNP
Volume
45
Issue
1
Year of publication
2000
Pages
49 - 51
Database
ISI
SICI code
1001-6538(200001)45:1<49:ULCSMT>2.0.ZU;2-X
Abstract
The level of lipid peroxidation and cellular necrosis in rat living brain s lices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent pr obes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (PI). T he hypoxia and/or reoxygenation injury in rat brain slices is markedly decr eased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.