Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti

Authors
Zhu, B Dai, XM Zhu, JB Yu, GQ Shen, SJ
Citation
B. Zhu et al., Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti, CHIN SCI B, 45(1), 2000, pp. 60-64
Citations number
14
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
10016538 → ACNP
Volume
45
Issue
1
Year of publication
2000
Pages
60 - 64
Database
ISI
SICI code
1001-6538(200001)45:1<60:RROTSD>2.0.ZU;2-B
Abstract
The 660 bp region between nodD3 P1 promoter and the following coding region of Rhizobium meliloti has been studied. This region is designated "downstr eam sequences". It consists of two potential open reading frames, ORF1 and ORF2. Studies on the role of the downstream sequences on the activity of no dD3 P1 with nod D3(P1)-lacZ fusion show that deletion of the sequences cont aining ORF2 causes the increase of the activity of the fusion; on the contr ary, addition of extra copies of ORF2 markedly decreases the activity of th e fusion. These results indicate that the product of ORF2 plays a negative role in the expression of nod D3.