M. Merino et al., Reduction of different nitro-compounds on electroenzymatic system: Cytochrome c reductase-NAD(P)H modified carbon paste electrode, ELECTROANAL, 11(17), 1999, pp. 1285-1292
A flavoenzyme cytochrome c reductase NAD(P)H mixture has been used to modif
y a carbon paste electrode. The modified electrode was characterized by cyc
lic voltammetry varying the scan rate and temperature. Cathodic and anodic
peaks were observed at -0.45 and -0.39 V, respectively, for a scan rate of
100 mV/s. The enzyme-electrode is found to reduce electrocatalytically nitr
oimidazole and nitrofurane compounds. However, this effect was not observed
on the reduction of nitrobenzene derivatives such as nitrobenzene substitu
ted 1,4-dihydropyridines. Conditions such as pH and percentage of DMF have
been optimized for the determination of these nitro-compounds. The best ele
ctrocatalytic reactions were obtained at pH 8.5, coincident with the optima
l activity of the enzyme.