Microorganisms indigenous to the bed sediments of a black-water stream util
ized 1,2-dichloroethene (1,2-DCE) as a sole carbon substrate for aerobic me
tabolism. Although no evidence of growth was observed in the minimal salts
culture media used in this study, efficient aerobic microbial mineralizatio
n of 1,2-DCE as sole carbon substrate was maintained through three sequenti
al transfers (10(7) final dilution) of the original environmental innoculum
. These results indicate that 1,2-DCE can be utilized as a primary substrat
e to support microbial metabolism under aerobic conditions.