Hemophilic NCAM interactions interfere with L1 stimulated neurite outgrowth

The cell adhesion molecules NCAM and L1 are considered to play key roles in neuronal development and plasticity. L1 has been shown to interact with NC AM, possibly through NCAM binding to oligomannosidic glycans present in L1, We investigated the effect of recombinant immunoglobulin (Ig) modules of N CA-IM involved in hemophilic NCAM binding, on L1 induced neurite outgrowth from PC12-E2 cells and found a complete inhibition of L1 induced neurite ou tgrowth after addition of Ig-modules 1, 2 and 3 of NCAM, suggesting that th e ligation state of NCAM is crucial for normal L1 signaling. (C) 1999 Feder ation of European Biochemical Societies.