High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome

Authors
Thomas, MG Bradman, N Flinn, HM
Citation
Mg. Thomas et al., High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome, HUM GENET, 105(6), 1999, pp. 577-581
Citations number
16
Categorie Soggetti
Molecular Biology & Genetics
Journal title
HUMAN GENETICS
ISSN journal
03406717 → ACNP
Volume
105
Issue
6
Year of publication
1999
Pages
577 - 581
Database
ISI
SICI code
0340-6717(199912)105:6<577:HTAO1M>2.0.ZU;2-M
Abstract
We describe an integrated approach to the determination of complex Y chromo some haplotypes that is both fast and relatively inexpensive. The method em ploys GeneScan technology to enable a researcher to assay repeat number var iation at ten microsatellite loci and determine the status of 11 diallelic polymorphisms. The method requires only four PCRs and four GeneScan runs pe r sample and is relatively insensitive to sample DNA concentration.