As a contribution to the study and preservation of indigenous wine yeast po
pulations, the A. A. initiated a programme of isolation and characterizatio
n of yeast strains from grapes of the Italian region of Marche. During the
1996 vintage, grape samples were collected From three groups of vineyards d
iffering in the pesticide treatments given, of the 279 yeasts isolated, thi
rty-nine were assigned to the species Saccharomyces cerevisiae. The majorit
y of the isolates and all thirty-nine 5. cerevisiae came from vineyards whi
ch had not been treated with systemic pesticides, indicating that these pes
ticides may exert a negative effect on the biodiversity of the grape microf
lora, and in particular on the occurrence of S. cerevisiae on grapes. The t
hirty-nine S. cerevisiae isolated were subjected to microfermentation trial
s, in which ten of them showed promising Fermentative capabilities.