Quantification of single solute and competitive adsorption isotherms usinga closed-loop perturbation method

A modification of the classical method of perturbation chromatography for m easuring isotherms of the adsorption of dissolved components is suggested. The general principle of the method consists in analyzing responses of the chromatographic system to small perturbations at different equilibrium conc entrations. Essential advantages of the method are: (a) only retention time s or volumes have to be measured and no detector calibration is required an d (b) experiments with mixtures can be performed and analyzed efficiently. The modification suggested in this paper is the application of a closed-loo p arrangement allowing the efficient exploitation of the sample. Experiment al data for four different chromatographic systems are presented to illustr ate the method. With the determined adsorption isotherms elution profiles c ould be predicted satisfactorily. (C) 1999 Elsevier Science B.V. All rights reserved.