Angiogenesis in neuroblastoma: Relationship to survival and other prognostic factors in a cohort of neuroblastoma patients

Purpose: To study angiogenesis in neuroblastoma, using morphometric and com puterized image analysis, and correlate the results with survival and other prognostic factors. Patients and Methods: Sixty-nine patients from the Spanish Cooperative Stud y for Neuroblastoma were studied. Tumoral angiogenesis was studied using an avidin-biotin immunoperoxidase technique with an anti-CD34 antibody. Vascu lar parameters (VPs) were analyzed by a computerized system. Statistical an alysis wets also performed. Results: Sixty-six samples had adequate tumoral tissue, and their tumoral v essels were counted. Endothelial cells were more prominent in pure neurobla stomas than in maturing and more mature tumors. VPs showed no statistical d ifference between the groups of patients as defined by the levels of the ot her prognostic factors in neuroblastoma: age, stage, histopathology, TRK-A, p-glycoprotein expression, or MYCN copy number. In patients who relapsed, tumors did not show statistically significant difference in VPs when compar ed with tumors from patients who did not relapse. There was also no differe nce in VPs in tumors from living patients when compared with tumors from de ceased patients. Overall survival was 75%, and event-free survival was 55% at 50 months. Conclusion: VPs could be adequately determined by a computerized system in neuroblastoma; however, VPs were not predictive of survival for our patient s. In our patients, neither disseminated nor local relapses were influenced by the angiogenic characteristics of the tumors. (C) 2000 by American Soci ety of Clinical Oncology.