A. Younossi-hartenstein et al., Embryonic development of the nervous system of the rhabdocoel flatworm Mesostoma lingua (Abildgaard, 1789), J COMP NEUR, 416(4), 2000, pp. 461-474
We have analyzed the embryonic development of the Mesostoma nervous system,
using a combination of histology, transmission electron microscopy, and wh
olemount immunohistochemistry. Neural progenitors are formed at an early st
age when the Mesostoma embryo constitutes a multilayered mesenchymal mass o
f cells, A neurectoderm as in vertebrates or arthropods is absent. Only aft
er neurons in the deep layers of the embryo have started differentiating do
superficial cells reorganize into an epithelium that will give rise to the
epidermis. Neurons are clustered in two anterior, bilaterally symmetric br
ain hemispheres. An antibody against acetylated beta-tubulin (anti-acTub) t
hat labels neurotubules reveals an invariant pattern of pioneer neurons in
the brain of midstage embryos. Pioneer neurons are grouped in several small
clusters at characteristic positions. They pioneer several commissural tra
cts of the brain and two pairs of ventral and dorsal connectives, respectiv
ely. (C) 2000 Wiley-Liss, Inc.