A. Udomkit et al., Molecular cloning of a cDNA encoding a member of CHH/MIH/GIH family from Penaeus monodon and analysis of its gene structure, J EXP MAR B, 244(1), 2000, pp. 145-156
Citations number
27
Categorie Soggetti
Aquatic Sciences
Journal title
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
We report the nucleotide and deduced amino acid sequences of Pem-CMG peptid
e, a member of crustacean CHH/MIH/GM peptide family, in black tiger prawn (
Penaeus monodon). The 5' and 3' fragments of Pem-CMG cDNA were cloned by th
e method of rapid amplification of cDNA ends (RACE). The two fragments cons
titute a combined cDNA length of 593 bp with a 77 bp overlapping region. Se
quence analysis reveals the presence of a 384 bp open reading frame which w
as subsequently cloned. The open reading frame encodes a precursor peptide
that is comprised of 128 amino acids, with a putative processing site, KR.
The mature peptide consists of 74 amino acid residues, the sequence of whic
h is significantly homologous to those of the CHH/MIH/GM family known from
other crustaceans. Analysis of a genomic fragment of Pem-CMG reveals a sing
le intron of 314 bp interrupting the coding sequence for the mature peptide
. The presence of only one intron in Pem-CMG gene suggests that this gene i
s structurally different from the previously reported MIN gene of Charybdis
feriatus and CHH-like gene of Metapenaeus ensis which possess two introns
in their coding sequences. (C) 2000 Elsevier Science B.V. All rights reserv
ed.