Characterisation of Borrelia burgdorferi sensu lato strains isolated from patients with skin manifestations of Lyme borreliosis residing in Slovenia

Lyme borreliosis is the most prevalent tick-borne infection in Slovenia, Sk in disorders are the most frequent clinical manifestations, The aim of the present study was to assess the phenotypic and genotypic diversity of a lar ge number of human Borrelia burgdorferi sensu late isolates and to evaluate any association between the isolates and different clinical manifestations . All 103 strains tested were from patients suffering from the skin disorde rs of Lyme borreliosis, Skin biopsies, cerebrospinal fluid and blood sample s from patients were inoculated into modified Kelly Pettenkofer medium. Pro tein profiles were determined by SDS-PAGE and species identification and pl asmid profiles by pulsed-field gel electrophoresis, MluI digestion profiles showed that 87 (84.5%) isolates belonged to B. afzelii, 15 (14.5%) to B. g arinii and 1 (1%) to B. burgdorferi sensu stricto, The number of plasmids i n each strain varied from three to seven, and the plasmid size ranged from 15 to 65 kb, Four isolates of B. garinii possessed multiple large plasmids and four isolates had a large plasmid dimer (three B. afzelii and one B. ga rinii), Isolates showed qualitative and quantitative differences in protein expression. The study found differences in the expression of OspB and OspC proteins between B, afzelii and B, garinii strains. OspB was expressed sig nificantly more often by B. afzelii (78 of 87, 89.6%) than by B. garinii (4 of 15, 26.6%) isolates, while OspC protein was expressed significantly mor e often by B. garinii (14 of 15, 93.3%) than by B. afzelii (51 of 87, 58.6% ) isolates. In Slovenia, B. afzelii causes the majority of skin lesions. Th e isolates investigated showed plasmid and protein diversity. Heterogeneity of the spirochaetes may be important for virulence, and may have implicati ons for pathogenesis and therapy of the infection. Differences in immunodom inant proteins also have an important impact on serological testing and vac cine development.