ENCAPSULATION OF FLAVODOXIN IN REVERSE MICELLES

Citation
S. Andrade et al., ENCAPSULATION OF FLAVODOXIN IN REVERSE MICELLES, Biochemical and biophysical research communications, 234(3), 1997, pp. 651-654
Citations number
17
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
234
Issue
3
Year of publication
1997
Pages
651 - 654
Database
ISI
SICI code
0006-291X(1997)234:3<651:EOFIRM>2.0.ZU;2-5
Abstract
The regulation of the properties of Desulfovibrio gigas flavodoxin in AOT/water/iso-octane micellar system was studied. UV-visible spectrosc opic studies have shown that photoreduction of flavodoxin in the prese nce of EDTA leads to hydroquinone formation through the intermediate s emiquinone. The [free FMN] - [bound to flavodoxin PMN] equilibrium (an d hence, the amount of apoprotein) depends on redox state of FMN and o n hydration degree which controls the micellar size. Thus, a new metho d of reversible cofactor removing under mild conditions (at low hydrat ion degree of micelles) is suggested, accompained by isolation of apo- form of the protein. (C) 1997 Academic Press.