J. Fulford et al., Experimental determination of the dependence of OH radical yield on photonenergy: A comparison with theoretical simulations, J PHYS CH A, 103(51), 1999, pp. 11345-11349
On the basis of simulations of water radiolysis, it has been postulated tha
t the yield of OH radicals, which become homogeneously distributed, is depe
ndent upon energy when irradiated with low-energy photons. The aim of this
study is to determine experimentally the dependence of the yield of OH radi
cals, which escape intratrack recombination, on photon energy of incident r
adiation using plasmid DNA as a probe. The yields of single strand breaks (
ssb) induced in plasmid DNA (pUC18) when irradiated with photons varying in
energy from 0.28 keV to 1.25 MeV was determined and, when normalized relat
ive to the yield for Co-60 irradiation, may be used as a measure of the yie
ld of OH radicals escaping radiation tracks. As the photon energy decreases
from 1.25 MeV to 1.5 keV the OH radical yield decreases from 0.290 to 0.07
2 mu mol J(-1), respectively, in line with an increased ionization density
of the radiation and hence an increasing probability of radical recombinati
on. However, with a further decrease in photon energy from 1.5 to 0.28 keV
there is an upturn in OH radical yields. Carbon-K X-rays are found to have
a significantly higher yield of 0.257 mu mol J(-1) than that associated wit
h the higher energies. The experimental dependence is compared with a numbe
r of theoretical calculations, which predict an upturn in OH radical yields
between 0.1 and 1 keV. Such a dependence of OH radical yields on energy pr
ovides experimental data suitable for direct comparison with simulations, a
iding their refinement and development.