This paper presents a nearest neighbor method for the spatial analysis of d
ata collected from discrete field sampling sites. The method was applied to
point counts of birds at permanent survey sites in the Nicolet National Fo
rest of northeastern Wisconsin. The spatial analysis method we developed us
es a Monte Carlo randomization approach to test for non-randomness not only
of the mean nearest neighbor distance between n points but also the mean s
econd nearest, third nearest,..., to (n-1)th nearest distances to reveal sp
atial information at multiple scales. Because the bird survey sites are not
randomly distributed throughout the forest, the survey sites at which a gi
ven species was recorded were compared with random samples drawn from the t
otal survey sites rather than from all possible points within the forest. M
ore refined analyses restricted the randomization by (a) habitat type, in o
rder to separate the effects of non-randomly distributed habitat types on s
pecies' distributions; and (b) north-south regions of the forest, in order
to account for regional gradients in distribution which were evident for so
me species. Spatial patterns among the sites at which the birds were detect
ed reveal information about the scale at which the birds are distributed in
their environment and provide a more complete picture of multi-scale bird
population dynamics.