Wp. Qian et al., Site-directed immobilization of immunoglobulin G on 3-aminopropyltriethoxylsilane modified silicon wafer surfaces, MAT SCI E C, 8-9, 1999, pp. 475-480
Immobilization and distribution properties of the polyclonal antibodies to
hepatitis B surface antigen (anti-HBsAg) onto 3-aminopropyltriethoxylsilane
(APTES) modified silicon wafer surfaces under two different kinds of coupl
ing conditions were investigated using atomic force microscopy (AFM). Enzym
e immunoassay (EIA) and X-ray photoelectric spectroscopy (XPS) were also us
ed as the complimentary techniques to quantify a NH2-terminated siloxane mo
nolayer and a antibody layer. Anti-HBsAg antibodies were site-directly immo
bilized onto the APTES modified surface: (1) in acetate buffer of pH 5.2 fo
r 3 days at 4 degrees C and (2) in acetate buffer of pH 5.2 containing 5 mM
NaBH4 for 12 h, via aldehydes generated on the carbohydrate side chains at
the C-terminal of IgG using periodate (NaIO4)-oxidized reactions. (C) 1999
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