A pyrene seco-pseudonucleoside in constructing interaction-sensitive fluorescent DNA probes

Authors
Korshun, VA Balakin, KV Proskurina, TS Mikhalev, II Malakhov, AD Berlin, YA
Citation
Va. Korshun et al., A pyrene seco-pseudonucleoside in constructing interaction-sensitive fluorescent DNA probes, NUCLEOS NUC, 18(11-12), 1999, pp. 2661-2676
Citations number
47
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
11-12
Year of publication
1999
Pages
2661 - 2676
Database
ISI
SICI code
0732-8311(1999)18:11-12<2661:APSICI>2.0.ZU;2-C
Abstract
Based on 4-(1-pyrenyl)-1,3-butanediol, a pyrene seco-pseudonucleoside, we p repared a nonnucleotide phosphoramidite reagent and a pyrene-modified LCAA- CPG-support and employed them in solid-phase synthesis of pyrene-labeled ol igonucleotides with varying numbers and positions of the pseudonucleoside r esidues. Changes in the fluorescence intensity and the spectral curve shape upon hybridization were observed that depended on the position(s) of the m odified unit(s) in the probe.