5 '-tailed octanucleotide primers for cycle sequencing

Citation
D. Loakes et al., 5 '-tailed octanucleotide primers for cycle sequencing, NUCLEOS NUC, 18(11-12), 1999, pp. 2685-2695
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
11-12
Year of publication
1999
Pages
2685 - 2695
Database
ISI
SICI code
0732-8311(1999)18:11-12<2685:5'OPFC>2.0.ZU;2-6
Abstract
Recently we reported the use of octamer primers tailed with 5-nitroindole f or use as primers in cycle sequencing reactions. Here we report the success ful use of some other universal base analogues to improve the effectiveness of an octamer sequencing primer. These analogues are 5-nitroindazole, 3-ni tropyrrole and benzimidazole.