Introduction of 6-formylcytidine into a Myb binding sequence

Authors
Kittaka, A Kuze, T Amano, M Tanaka, H Miyasaka, T Hirose, K Yoshida, T Sarai, A Yasukawa, T Ishii, S
Citation
A. Kittaka et al., Introduction of 6-formylcytidine into a Myb binding sequence, NUCLEOS NUC, 18(11-12), 1999, pp. 2769-2783
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
11-12
Year of publication
1999
Pages
2769 - 2783
Database
ISI
SICI code
0732-8311(1999)18:11-12<2769:IO6IAM>2.0.ZU;2-A
Abstract
Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 me rs) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC-5 '. The modified duplex showed Tm of 67 degrees C, which was six degrees low er than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and th e binding was almost completely abolished.