Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity

Authors
Farrer, RA Previte, MJR Olson, CE Peyser, LA Fourkas, JT So, PTC
Citation
Ra. Farrer et al., Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity, OPTICS LETT, 24(24), 1999, pp. 1832-1834
Citations number
8
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science","Optics & Acoustics
Journal title
OPTICS LETTERS
ISSN journal
01469592 → ACNP
Volume
24
Issue
24
Year of publication
1999
Pages
1832 - 1834
Database
ISI
SICI code
0146-9592(199912)24:24<1832:SDWATF>2.0.ZU;2-R
Abstract
We describe a laser-scanning two-photon fluorescence microscope that is cap able of observing single molecules with excellent temporal resolution and t hree-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present. single-molecule fluorescence data obtained in sever al different scanning modes. In addition, a polarization-sensitive detectio n scheme can provide detailed three-dimensional information about the orien tations of molecules in any of these scanning modes. (C) 1999 Optical Socie ty of America OCIS codes: 180.2520, 190.4180.