Interaction trap experiment with CDC6

CDC6 is an essential gene of yeast Saccharomyces cerevisiae. Although DNA s equence of the gene is available for a long time, biochemical function of C dc6 protein in the cell cycle remains unclear. Using the interaction trap e xperiment we were looking for proteins interacting specifically with Cdc6. Four gene products interacting with Cdc6 were detected. By sequence analysi s we found that ECM11 codes for the protein involved in the cell wall synth esis, YNL201 codes for the protein of unknown function, probably involved i n the carbon metabolism, YOR279 codes for protein of completely unknown fun ction with no significant similarity with any known protein, and the intera ction with Ty1 retrotransposition element was also found The strongest inte raction with Cdc6 bait measured as beta-galactosidase activity was observed with ECM11 and YNL201; YOR279 interacts slightly weaker. The weakest beta- galactosidase activity was obtained by Ty1A element. The strongest suppress ion of cdc6-1 mutation was observed by Ty1A element, the slight one with EC M11 and YNL201 but no suppression of thermosensitive mutation was detected for YOR279.