PREPARATION AND CRYSTALLIZATION OF A CROSS-LINKED COMPLEX OF BOVINE ADRENODOXIN AND ADRENODOXIN REDUCTASE

Citation
A. Lapko et al., PREPARATION AND CRYSTALLIZATION OF A CROSS-LINKED COMPLEX OF BOVINE ADRENODOXIN AND ADRENODOXIN REDUCTASE, Proteins, 28(2), 1997, pp. 289-292
Citations number
28
Categorie Soggetti
Biology
Journal title
ISSN journal
08873585
Volume
28
Issue
2
Year of publication
1997
Pages
289 - 292
Database
ISI
SICI code
0887-3585(1997)28:2<289:PACOAC>2.0.ZU;2-8
Abstract
Bovine adrenodoxin was cross-linked to adrenodoxin reductase with 1-et hyl-3-(3-dimethyl-aminopropyl) carbodiimide. Mass spectrometry showed the reaction product to be a 1:1 complex of the two proteins with M-r = 64,790 +/- 50. The cross-linked complex showed cytochrome c reductas e activity and could be crystallized by hanging-drop vapor diffusion. Crystals of the adrenodoxin-adrenodoxin reductase complex are hexagona l, space group P6(1)22 or P6(5)22, with a = 93.26 Angstrom and c = 612 .20 Angstrom and diffract to 2.9 Angstrom resolution at 100 K. Assumin g two cross-linked complexes per asymmetric unit yields a reasonable V -M of 2.97 Angstrom(3)/Da. (C) 1997 Wiley-Liss, Inc.