PURIFICATION AND CRYSTALLIZATION OF PSEUDOMONAS-AERUGINOSA CHLORAMPHENICOL ACETYLTRANSFERASE

Authors
TIAN Y BEAMAN TW RODERICK SL
Citation
Y. Tian et al., PURIFICATION AND CRYSTALLIZATION OF PSEUDOMONAS-AERUGINOSA CHLORAMPHENICOL ACETYLTRANSFERASE, Proteins, 28(2), 1997, pp. 298-300
Citations number
12
Categorie Soggetti
Biology
Journal title
Proteins → ACNP
ISSN journal
08873585
Volume
28
Issue
2
Year of publication
1997
Pages
298 - 300
Database
ISI
SICI code
0887-3585(1997)28:2<298:PACOPC>2.0.ZU;2-S
Abstract
A chloramphenicol acetyltransferase from Pseudomonas aeruginosa genomi c DNA has been overexpressed, refolded, purified, and crystallized. Cr ystals suitable for a three-dimensional x-ray structure determination were obtained from solutions of polyethyleneglycol methyl ether 2000 c ontaining NiCl2 at pH 8.5. These crystals belong to the cubic space gr oup P4(1/3)32 (a = 154.8 Angstrom) and diffract x-rays to similar to 3 .2 Angstrom resolution. (C) 1997 Wiley-Liss, Inc.