Application of liquid pre-column capillary electrophoresis technique to the study of interaction between drug enantiomers and human serum albumin

Based on the chiral separation of several basic drugs, dimetindene, tetryzo line, theodrenaline and verapamil, the liquid pre-column capillary electrop horesis (LPC-CE) technique was established. It was used to determine free c oncentrations of drug enantiomers in mixed solutions with human serum album in (HSA). To prevent HSA entering the CE chiral separation zone, the mobili ty differences between HSA and drugs under a specific pH condition were emp loyed in the LPC. Thus, the detection confusion caused by protein was total ly avoided. Further study of binding constants determination and protein bi nding competitions was carried out. The study proves that the LPC technique could be used for complex media, particularly the matrix of protein coexis ting with a variety of drugs.