This paper describes the fabrication and operating principles of a reconfig
urable diffraction grating based on a microfluidic device. The device consi
sts of an array of microscopic channels (50 mu m wide and 20 mu m deep) def
ined by the conformal contact between a transparent elastomeric material th
at has an embossed surface relief and a flat glass substrate. The microchan
nels can be filled reversibly with fluids (gas, aqueous solutions, or organ
ic solutions). The difference in index of refraction between the fluid in t
he array of microchannels and the structural elastomeric solid generates a
difference in,the phase of the light passing through the device; absorption
by the fluid changes the amplitude of the light. Both of these effects giv
e rise to diffraction. The gratings are reconfigured by pumping liquids wit
h different indices of refraction and optical densities through the microch
annels. In these experiments, the devices exhibited maximum depths of modul
ation of similar to 20 dB and switching times of similar to 50 ms; the volu
me of liquid sampled by the incident light was about 8 nl. The potential ap
plication of these devices as sensing elements in micro total analysis syst
ems (mu TAS) and as actuators is evaluated. (C) 1998 Elsevier Science S.A.
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