Biosensors for the determination of environmental inhibitors of enzymes

Characteristic features of operation and practical application of enzyme bi osensors intended for determination of enzyme inhibitors referred to the co mmon environmental pollutants. The influence of the way of detection of the enzymatic reaction, the method of enzyme immobilization the composition of the reaction medium on the analytical characteristics of determination of inhibitors is considered. Published data on the design of biosensors for th e detection of pesticides and heavy metals are summarised. Primary attentio n was denoted to cholinesterase biosensors. The approaches to the estimatio n of kinetic parameters of inhibition are considered and factors influencin g the selectivity and sensitivity of inhibitor determination in environment al. objects are considered. The potentialities and limitations of the detec tion of environmental pollutants in real samples and in held conditions are discussed.