Mc. Cerquetti et Mm. Gherardi, Vaccination of chickens with a temperature-sensitive mutant of Salmonella enteritidis, VACCINE, 18(11-12), 2000, pp. 1140-1145
One-day old chickens were inoculated with temperature-sensitive mutant E/1/
3 of S. enteritidis. Two routes of inoculation were used: oral and intraper
itoneal (ip). One group of chickens were given two oral inoculations (oral-
oral). A second group received two ip inoculations (ip-ip). A third group r
eceived the first dose orally and the second ip (oral-ip) and the fourth gr
oup was given the first dose ip and the second dose orally (ip-oral). The v
accine strain was safe even when inoculated at high doses, and induced stro
ng protection against virulent S. enteritidis strain after oral challenge.
Results show that vaccination with mutant E/1/3 reduced the number of anima
ls shedding the pathogen after challenge. Furthermore, animals immunized or
al-oral and oral-ip showed a significant reduction in cecal and spleen colo
nization by virulent Salmonella. (C) 2000 Elsevier Science Ltd. All rights
reserved.