Effect of batroxobin against dog heart ischemia/reperfusion injury

AIM: To study the effect of batroxobin(Bat) on dog heart ischemia/reperfusi on (VR) injury. METHODS: Dog heart. VR injury was induced by occluding the left anterior descending coronary artery for 30 min and restoring blood per fusion for 90 min. Bat was intravenously injected before heart ischemia and 15 min before reperfusion. Plasma creatine kinase (CX), lactate dehydrogen ase (LDH), and myocardial malondiadehyde (MDA) concentrations were measured . The pathologic changes of VR myocardium were observed. RESULTS: Bat reduc ed the mortality rate of I/R dog (VR group 65.0 % vs Bat- I group 30.0 % an d Bat- group 28.6 %, P < 0.05). Myocytes of I/R heart showed intracellular edema, damaged mitochondria, and concentrated nucleus. Bat decreased these changes. In Bat- I and Bat-II group, plasma CK and LDH level were reduced, the + dp/dt(max) and - dp/ dt(max) at 30 min after ischemia and 90 min afte r reperfusion were elevated, and left ventricular end dilation pressure(LVE DP) was lowered. The myocardial MDA contents were decreased by 42.3 % and 3 8.1 % (P < 0.01) in Bat- I and Bat- II group, respectively. CONCLUSION: Bat may exert an apparent role against dog heart ischemia/reperfusion injury a nd improve myocardial function.