Study on facial motoneuronal death after proximal or distal facial nerve transection

Background: Among the cranial nerves, the facial nerve is most liable to be damaged. Pathologic changes in the facial motor nucleus (FMN) after nerve injury are not well recognized, and the optimal time for facial nerve recon struction after axotomy is controversial. In this study, to clarify the pat hologic change in the FMN after axotomy and to determine the best time for surgery, facial motoneuronal death was investigated after facial nerve inju ry. Methods: Sixty Wistar rats were divided into proximal and distal groups. In the proximal group, the right facial nerve was transected at the porus of internal acoustic meatus. In the distal group, the nerve was cut at the sty lomastoid foramen. The animals were sacrificed from day 3 to day 60 after s urgery, and the brainstem was fixed with 4% paraformaldehyde. The FMN were then examined in serial sections stained with Cresyl Violet, and facial mot oneurons were counted under a light microscope. Results: The death rate of facial motoneurons in the animals that underwent proximal axotomy was found to be higher and cell death occurred earlier than in the distal axotomy an imals at every time point. Moreover, neuron death increased with time and p eaked at 15 days after surgery. Conclusions: The results indicate that the injury site was correlated with facial motoneuronal death, and suggest that reconstructive surgery should b e performed as early as possible.