Optimization of the experimental parameters in the determination of rifamycin SV by adsorptive stripping voltammetry

A procedure for the determination of rifamycin SV by adsorptive stripping v oltammetry (AdSV) has been optimized. Two different voltammetric techniques , namely differential pulse adsorptive stripping voltammetry (DPAdSV) and s quare wave adsorptive stripping voltammetry (SWAdSV) were used. In order to determine the most sensitive technique, an optimization of the experimenta l parameters was carried out for every technique using experimental design methodology. A canonical analysis of the response surface was carried out, which was adjusted to determine the optimum and the influence of the experi mental factors. When this lay outside the ambit of the experiment, the opti mum path method was used for the optimising process. The detection limits o btained, 3.108 x 10(-8) mol dm(-3) for DPAdSV and 1.233 x 10(-8) mol dm(-3) for SWAdSV, show that SWAdSV is a more sensitive technique for the analysi s of rifamycin SV. Finally, this method was used to determine rifamycin SV in real samples. (C)2000 Elsevier Science B.V. All rights reserved.