Tungsten determination in rat and dog plasma samples by inductively coupled plasma emission spectrometry - Application to preclinical pharmacokineticstudies

This paper describes an inductively coupled plasma emission spectrometric m ethod for the analysis of tungsten in dog and rat plasma. Calibration curve s were obtained in the range 134-1300 ng/ml. The relative standard deviatio n ranged from 0.4 to 17%, and accuracy was between 89 and 105%. Dilution ha s no influence on the performance of the method which could then be used to quantitate plasma samples containing up to 90 mu g/ml. The Limit of quanti fication was 100 ng/ml, at this level, the average relative standard deviat ion was 17%. The results obtained indicate that the method described for as saying tungsten in plasma samples fulfills the accuracy and precision requi rements necessary to carry out pharmacokinetic studies. This method was use d to determine pharmacokinetic parameters in rats and dogs. (C) 2000 Elsevi er Science B.V. All rights reserved.