Enhanced sterol-acyl transferase activity promotes sterol accumulation in Saccharomyces cerevisiae

The sterol-acyl transferase encoded by the gene ARE2 was transcriptionally deregulated in the yeast Saccharomyces cerevisiae to understand its role in sterol storage and sterol enrichment. Our results show that sterols can in deed be enriched in yeast by enhancing the capacity of the cells to esterif y sterols, ARE2 overexpression had no impact on the accumulation of the ear ly sterols such as lanosterol, but influenced the later intermediates and t he end product ergosterol. Thus an enhanced conversion of free sterols to t heir esterified counterparts may provide a tool to increase the overall ste rol content of the yeast cell. We have previously shown that the overexpres sion of a truncated version of the key enzyme of the early sterol pathway, HMG-CoA reductase (HMG1), leads to an increase in the early sterols such as lanosterol and zymosterol. The simultaneous deregulation of both genes in one strain produces a cumulative effect in that both early and late sterols are enhanced. Karmellae-like structures can be detected when Are2p is over expressed. Are2p therefore constitutes a new member of the karmellae-induci ng protein family.