Nutritional requirements for the production of pyrazoloisoquinolinone antibiotics by Streptomyces griseocarneus NCIMB 40447

This paper describes the effect of different nutrients on the production of pyrazoloisoquinolinone antibiotics (APHE) by Streptomyces griseocarneus. I n a chemically defined medium with glucose as carbon and L-lysine as nitrog en source all APHE antibiotics (APHE-1 to -3) are produced, APHE-3 being th e most abundant. Propionate and butyrate used as precursors with glucose as main carbon soul-ce increased the production of APHE-1 and -2, respectivel y. The presence of propionate or butyrate reduced the production of APHE-3. Results obtained in minimal medium supplemented with l-valine and L-histid ine indicate a relationship between these amino acids and APHE biosynthesis . These data, together with those obtained in the presence of precursors of fatty acids, also show possible links with fatty acid biosynthesis. Differ ent nutritional requirements were found for APHE-3 production in comparison with APHE-1 and APHE-2.