We propose an empirical sulphur abundance determination method based o
n the strong sulphur emission lines, [S II] lambda lambda 6716, 6731 a
nd [S III] lambda lambda 9069, 9531 in H II regions. From a compilatio
n of literature data we have made a calibration of sulphur abundance v
ersus ([S II] + [S III])/H beta, similar to what has been widely used
for the more easily observable iso-electronic element oxygen. This ena
bles abundance determinations in extragalactic H II regions without me
asurements of weak temperature sensitive lines for use in model calcul
ations. As a first application of the empirical calibration 15 spectra
covering the wavelength range 3650-10000 Angstrom have been obtained
of H II regions at various galactocentric distances in the spiral gala
xy NGC 300, by employing specially designed multiaperture slitmasks wh
ich allow simultaneous observations of several H II regions. Sulphur a
nd oxygen abundances are determined and an oxygen gradient in agreemen
t with previously published work is found as well as a somewhat steepe
r sulphur abundance gradient. This results in a slight decrease of log
S/O with increasing radius. The quite low values of S/O seem to confi
rm the trend demonstrated by Diaz et al. (1991) of a decrease in log S
/O at high metallicities, but still more data are needed before any de
finite conclusions can be drawn. We find as expected that the radiatio
n softness parameter eta decreases with increasing radius and the slop
e is comparable to what is found for spiral galaxies of similar metall
icity.