Permeation of hesperidin glycosides across Caco-2 cell monolayers via the paracellular pathway

The intestinal permeability to hesperidin glycosides was investigated by us ing a cultured monolayer of Caco-2 as a model for the small intestinal epit helium. Hesperidin glycosides were added to the apical side of the monolaye r, and the substances that permeated to the basolateral side were determine d by HPLC. Whereas hesperidin did not permeate across the Caco-2 monolayer, probably owing to its low solubility, the hesperidin glycosides did permea te. The transepithelial transport of hesperidin glycosides occurred in time - and dose-dependent manners. The transport was observed to be energy-indep endent, and was inversely correlated with the transepithelial electrical re sistance (TEER) of the monolayer. These results suggest that hesperidin gly cosides permeate across the Caco-2 cell monolayer via the paracellular path way.