Staphylococcal alpha-toxin provokes coronary vasoconstriction and loss in myocardial contractility in perfused rat hearts - Role of thromboxane generation
U. Sibelius et al., Staphylococcal alpha-toxin provokes coronary vasoconstriction and loss in myocardial contractility in perfused rat hearts - Role of thromboxane generation, CIRCULATION, 101(1), 2000, pp. 78-85
Citations number
37
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Background-Cardiac performance is severely depressed in septic shock. Endot
oxin has been implicated as the causative agent in Gram-negative sepsis, bu
t similar abnormalities are encountered in Gram-positive sepsis. We investi
gated the influence of the major exotoxin of Staphylococcus aureus, staphyl
ococcal alpha-toxin, in isolated perfused rat hearts.
Methods and Results-alpha-Toxin 0.25 to 1 mu g/mL caused a dose-dependent i
ncrease in coronary perfusion pressure that more than doubled. In parallel,
we noted a decrease in left ventricular developed pressure and the maximum
rate of left ventricular pressure rise (dP/dt(max)), dropping to a minimum
of <60% of control. These changes were accompanied by a liberation of thro
mboxane A(2) and prostacyclin into the coronary effluent. The release of cr
eatine kinase, lactate dehydrogenase, potassium, and lactate did not surpas
s control heart values, and leukotrienes were also not detected. Indomethac
in, acetylsalicylic acid, and the thromboxane receptor antagonist daltroban
fully blocked the alpha-toxin-induced coronary vasoconstrictor response an
d the decrease in left ventricular developed pressure and dP/dt(max) wherea
s the lipoxygenase inhibitor nordihydroguaiaretic acid, the platelet activa
ting factor antagonist WEB 2086, and the alpha-adrenergic antagonist phento
lamine were entirely ineffective. Inhibition of nitric oxide synthase even
enhanced the alpha-toxin-induced increase in coronary perfusion pressure an
d the loss in myocardial performance.
Conclusions-Purified staphylococcal alpha-toxin provokes coronary vasoconst
riction and loss in myocardial contractility. The responses appear to be la
rgely attributable to the generation of thromboxane and are even enhanced w
hen the endogenous nitric oxide synthesis is blacked. Bacterial exotoxins,
such as staphylococcal a-toxin, may thus be implicated in the loss of cardi
ac performance encountered in Gram-positive septic shock.