High resolution molecular typing of HLA class II region in the population of the island of Krk, Croatia

The HLA class II alleles (DRB1, DRB3, DRB5, DQA1, and DQB1) and haplotypic associations were studied in the population of the island of Krk using the PCR-SSOP method and the 12th International Histocompatibility Workshop prim ers and probes. Allele and haplotypic frequencies were compared with the ge neral Croatian population. Significant differences were observed between th e population of the island of Krk and Croatians for: a) three broad specifi cities at DRB1 locus (DRB1*01, *15, and *07), b) one allele at DRB3 locus ( DRB3*0301), c) one allele at DQA1 locus (DQA1*0201), d) one allele at DQB1 locus (DQB1*0303). Four unusual haplotypic associations, which have not yet been, described in the Croatian population, DRB1*1301-DQA1*0103-DQB1*0607, DRB1*1302-DQA1*0102-DQB1*0605, DRB1*1305-DQA1*0102-DQB1*0605 and DRB1*1305 -DQA1*0103-DQB1*0603 were observed in the population from the island of Krk .