Functional genomics and DNA array techniques in atherosclerosis research

DNA arrays are revolutionizing the analysis of gene expression and single n ucleotide polymorphisms of genomic DNA, Currently, the expression of 10-15% of human genes can be analysed simultaneously in a single experiment using cDNA or oligonucleotide-based format of DNA array. Alternatively, smaller DNA arrays with a limited number of selected genes, such as cytokines, grow th factors or transcription factors, can be used. In concordance with Human Genome Project, after a few years, the DNA arrays will allow the analysis of expression of the whole human genome and will have a great impact on bas ic research, drug development and diagnostics. It is important to character ise mechanisms of atherosderosis-related diseases at the level of gene expr ession so that new therapeutic strategies can be identified. With the aid o f DNA array it is possible to identify multiple, simultaneous, transcriptio nal events that ameliorate or contribute to atherogenesis, The results are non-physical maps of the function, hierarchy and interactions of genetic pr ograms. In this review we focus on DNA array technology and its application s in atherosclerosis research. Curr Opin Lipidol 10:515-519. (C) 1999 Lippi ncott Williams & Wilkins.