Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database

We propose a prediction method for human full-length cDNA by comparing sequ ence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is sele cted by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA, The accuracy of the predicted human full-length coding re gion is 83.3%, and the false positive rate is 16.7%, Five human full-length proteins out of 20 proteins are correctly predicted. (C) 1999 Federation o f European Biochemical Societies.