Am. Joussen et al., Effect of thalidomide and structurally related compounds on corneal angiogenesis is comparable to their teratological potency, GR ARCH CL, 237(12), 1999, pp. 952-961
Citations number
33
Categorie Soggetti
Optalmology
Journal title
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
Neovascular diseases are the leading causes of blindness in humans. Althoug
h several promissing compounds have been isolated, pharmacological treatmen
t remains difficult. Thalidomide has inhibitory effects on angiogenesis in
the corneal micropocket assay. However, the results vary considerably depen
ding on the administration route and ani mal model. The aim of this study,
therefore, was to investigate thalidomide and two of its derivatives, supid
imide and EM12, in the rabbit corneal micropocket assay. Using both basic f
ibroblast growth factor and vascular endothelial growth factor for initiati
on of the neovascular response, we were able to show a significant inhibiti
on of neovascularisation with all three substances. EM12, the most teratoge
nic derivative analysed, was demonstrated to be the most potent inhibitor o
f angiogenesis in this model. Thalidomide and supidimide did not show syste
mic side effects in the applied dosage. An equal dosage of EM12, however, r
esulted in significant weight loss of the animals, but did not increase ang
iogenic activity compared with lower doses. Together with earlier findings,
these data support a strong correlation between the antiangiogenic potenti
al and the teratogenic activity of thalidomide and structurally related com
pounds.